BACTERIAS DEGRADADORAS DE HIDROCARBUROS AISLADAS EN SUELOS CONTAMINADOS DE PETROLEO Aislar cepas bacterianas y. Aislamiento de bacterias potencialmente degradadoras de petróleo en hábitats de ecosistemas costeros en la Bahía de Cartagena, Colombia. Download Citation on ResearchGate | SELECCIÓN DE BACTERIAS CON CAPACIDAD DEGRADADORA DE HIDROCARBUROS Estudio y selección de bacterias aerobias degradadoras de hidrocarburos del petróleo aisladas de costas.
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They were bacterixs identified as members of the Bacillus, Alcaligenes, Pseudomonas, Acinetobacter, Marinomonas, Kurthia and Azotobacter genera. F9S and Bacillus sp. Taxonomic and metabolic variety notwithstanding, individual species seldom have the bactreias enzymatic toolset required to completely degrade the main organic compounds contaminating the ecosystem at any given time, and thus biodegradation usually proceeds through the concerted action of mixed populations or microbial consortia.
Online J Biol Sci. Total petroleum hydrocarbon TPH degradation was evaluated through SARA analysis, employing gas chromatography with an FID detector and infrared spectroscopy to analyze the aliphatic and aromatic hydrocarbon fractions, respectively.
Microbial degradation of hydrocarbons in the environment. At the end, 18 strains representing each unique combination of these parameters were chosen to be subjected to the selection process. Aliphatic hydrocarbons decreased in comparison with those of the abiotic degdadadoras regarding the non-resolved background cycloalkanes, resins and asphaltenes. Several authors have pointed out that short chain aliphatic hydrocarbons usually volatilize during the first hours after a spill.
A Mycobacterium strain with extended capacities for degradation of gasoline hydrocarbons. The concentration of associated hydroxyl groups OH -cm -1 increased for all treatments, as did that of carboxylic groups cm Bacterial community dynamics and hydrocarbon degradation during a field-scale evaluation of bioremediation on a mudflat beach contaminated with buried oil.
Finer taxonomic classification to the level of species was not pursued since, despite the existence of taxonomic identification schemes for microorganisms from marine ecosystems [12, 15], successfully identifying recently described genera and species of marine bacteria requires the application of molecular methods .
Each chart depicts both the profile of the strain under examination and that of the standard sample.
In general, biodegradation is expected to be more extensive in aliphatic hydrocarbons, which are far more amenable to this process than their aromatic counterparts [35, 47, 48].
Viewed as a whole, the latter possess the necessary genetic information to produce all the enzymes required to completely degrade complex hydrocarbon mixtures in damaged areas [4, 5]. Bioremediation potential of terrestrial fuel spills. F9S exhibited the best results regarding the degradation of asphaltenes. Rapid mineralization of benzo[a]pyrene by a microbial consortium growing on diesel fuel.
Hydrocarbons are lipophilic compounds that inhibit growth when present at high concentrations , inducing the bacterial stress response and a series of changes at the membrane, enzyme and protein levels [44, 46].
Effects of heavy fuel oil on the bacterial community structure of a pristine microbial mat. Taxonomic scheme for the identification of marine bacteria. Morphological descriptions were based on bacterial shape, motility and pigmentation. Measures taken after the Exxon Valdez spill included the addition of nutrients in the form of fertilizers Inipol EAP 22 and Customblerwhich increased the rate of oil removal by three-fold .
Strain selection All 33 isolated strains were grouped according to culture, degravadoras, physiological and biochemical ed. Two of the strains were also eegradadoras to decrease C Usually found at low concentrations in non-contaminated areas, their populations bloom in chronically contaminated environments .
Since hydrocarbon determinations were performed solely at the end of the study day 45no data are available to evaluate the biodegradation process in earlier time points.
No statistically significant differences were found for this parameter batcerias the examined strains, although the increased proportion of carboxyl and hydroxyl groups demonstrates the presence of biological oxidation processes.
The organic phase of the samples was extracted with 45 mL of HPLC-grade dichloromethane three extractions with a volume of 15 mL each using the liquid-liquid method for 30 min in a separating funnel, filtering the obtained organic extract through anhydrous reagent grade sodium sulfate.
Kurthia is a genus of Gram-positive bacilli described as environmental bacteria, whereas Azotobacter is a typical inhabitant of water bodies and soils. The degradation potential exhibited by these new isolates warrants further studies on their possible application to decontaminate coastal environments affected by oil spills. A microbiological survey of the western Cuban continental shelf found this species in both northern and southern locations .
Had hydrocarbon composition been determined at that point, we would have degradadorqs likely found practically identical levels of asphaltenes and resins in crudes treated with the strains under examination and in their controls, significantly decreased levels of saturated hydrocarbons in the former, and only a small drop in the concentration of aromatic hydrocarbons, since the bulk of their degradation takes place after 21 days.
Depth-resolved quantification of anaerobic toluene degraders and aquifer microbial community patterns in distinct redox zones of a tar oil contaminant plume. Field evaluations of marine oil spill bioremediation.
Published reports indicate that they can also degrade phenanthrene in soils  as well as anthracene, phenol  and methyl methylbromide in marine environments . Received in October, Enhanced biodegradation of Casablanca crude oil by a microbial consortium in presence of a rhamnolipid produced by Pseudomonas aeruginosa AT The cultures were discarded degradadooras 15 days.
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